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| Extraction and purification of cellulase fromwaste Pleurotus ostreatus compost |
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| Abstract: |
| In order to take full use of waste resources，the waste Pleurotus ostreatus compost was selected to extract
and process high-purity cellulase by orthogonal optimization，ammonium sulfate precipitation，dialysis and aqueous
two-phase extraction method. Results showed that the specific activity，purification fold and recovery rate of the resultant
enzyme was 19 705.60 U/mg，5.34 and 60.9%，respectively，after using 0.2 mol/L acetic acid-sodium acetate as buffer
solution（pH 5.5），ratio of material to liquid at 1∶4，immersing for 1-2 h at 15℃ and extraction by aqueous twophase
system composed of PEG6000 and ammonium sulfate with mass fraction of 20%. |
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