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| Extraction and scavenging free radical effects ofpolysaccharides from Gastrodia elata |
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| Abstract: |
| Response surface methodology（RSM）was applied to optimize the extraction conditions for
polysaccharides from Gastrodia elata based on single factor tests. Technological condition parameters were determined
according to regression analysis. The optimum conditions were as follows：ratio of water to material was 40：1，ultrasonic
power output was 75.70%，extraction time was 29.32 min. Spectrophotometry and chemiluminescence were employed to
compare the abilities of scavenging free radicals including DPPH·，O2
-·，·OH，H2O2 and ONOO- of polysaccharides
extracted from G. elata and three natural antioxidants α-lipoic acid，Vc and VE. The results showed that at the
concentration of 0-500 μg/mL，the scavenging ability of polysaccharides extracted from G. elata on DPPH· was similar
to that of α-lipoic acid，stronger than that of VE，obviously weaker than that of Vc，the highest clearance rate reached
12.23%；the scavenging ability on ·OH was weaker than that of Vc，similar to that of α-lipoic acid and VE at the
concentration of 400-500 μg/mL，the highest clearance rate reached 4.85%；the scavenging ability on H2O2 was stronger
than that of α-lipoic acid，slightly weaker than that of VE，obviously weaker than that of Vc，the highest clearance rate
reached 25.80%；the scavenging ability on ONOO- was obviously weaker than that of Vc and α-lipoic acid，the highest
clearance rate reached 20.52%. |
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