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| Cloning and expression analysis of cold induced proteinkinase gene CIPK07g in rice（Oryza sativa L.） |
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| Abstract: |
| The gene CIPK07g in rice was cloned，which was one of the members of Ca2+-dependent protein kinase
superfamily，and it’s signal peptide，transmemberane domain，conserved domain，expression profile in different
organisms and low temperature treatment were analyzed. The results showed that，the cDNA full length of CIPK07g gene
was 1 891 bp，including 279 bp 5′UTR and 259 bp 3′UTR，which encoded a polypeptide with 450 amino acids. There
was a characteristic conserved domain of CIPK gene family in CIPK07g protein，and the protein was a hydrophilicity
protein which included a transmemberane domain but had no signal peptide in N-terminal analyzed by bioinformatic
tools. The CIPK07g gene was highly expressed in leaf after 24 h cold treatment. |
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