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Identification of pathogen causing corynesporaleaf spot of tobacco in Jiangxi |
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Abstract: |
In order to identify the pathogen of flue-cured tobacco corynespora leaf spot disease in Ji’an,Jiangxi
Province,the fungi was isolated from diseased tobacco leaves. Based on the pathogenicity,morphological characteristics
and the sequence of ribosomal DNA -ITS,the pathogen was identified as Corynespora cassiicola,and the homology
analysis showed the sequence of ribosomal DNA -ITS isolated from the strains had homology of more than 99% with
Corynespora cassiicola published in GenBank. Finally,the pathogen was indicated as Corynespora cassiicola(Berk. &
Curt.)Wei. The results of biological characteristics showed that fungus couldn’t survive below 9℃ or above 39℃,grew
best at 26.5℃. It produced the most spores at 29℃,and the conidia germination was the most at 26.5℃. The disease is
the first report in Jiangxi,China. |
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