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Separation and purification of polyphenols fromareca inflorescence by macroporous resin |
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Abstract: |
Macroporous resins were used to separate and purify polyphenols from the extract of areca
inflorescence,and the conditions of separation and purification were determined. The adsorption capacity and desorption
rate of six kinds of resins,SP850,XAD-7HP,SP700,D101 and HP2MG,were compared to select the most suitable
one,through static adsorption experiment and dynamic adsorption and desorption experiment. The results showed
that AB-8 resin was the best one and the kinetic data fitted the pseudo-second-order kinetics model better. Optimal
conditions were as follows:the sample pH was 4.0,concentration of ethanol used as elution agent was 60%,initial
sample concentration was 0.4 mg/mL,adsorption rate and desorption rate was 3 BV/h. AB-8 resin performed great in
separating and purifying polyphenols from areca inflorescence,and the purification of polyphenols was 2.7% at first and
increased to 34.6% after purification. |
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