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| Isolation and cryopreservation techniques ofPseudorasbora parva blastomeres |
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| Abstract: |
| To…study…the…isolation…and…suitable…cryopreservation…of…Pseudorasbora parva blastomeres， comparison…
experiments…were…conducted…in…taking…off…the…egg…membrane…of…P. parva…blastula…with…different…concentrations…of…trypsase…
at…25℃， and…in…cryopreservation…for…P. parva blastomeres…with…different…protectants…and…at…different…temperatures.…Results…
showed…that…the…blastula…stage…of…P. parva was…achieved…in…120…min…after…fertilization， under…the…condition…of…microstream…
at…20℃.…It…took…12-18…min…to…take…off…the…egg…membrane…with…0.25%…trypsase…at…25℃， furthermore， the…survival…rate…of…
isolated…blastomeres…was…above…90%.…10%…DMSO+DMEM…was…used…as…a…tprotectant…at…-80℃， the…blastomeres…survival…
rate…was…above…50%…after…8…d； at…-196℃， the…survival…rate…was…above…70%…after…8…d， and…the…survival…rate…was…also…above…
60%…after…128…d.…The…result…indicated…that…better…condition…for…taking…off…the…egg…membrane…was…treatment…with…0.25%…
trypsase…for…12-18…min…at…25℃.…Higher…blastomeres…survival…rate（above…60%）could…be…maintained…at…-196℃…and…
10%…DMSO+DMEM. |
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