|
| Biochemical and cellular immunological responses of Pacificwhite shrimp， Litopenaeus vannamei to cold shock |
| |
| DOI： |
|
| Hits: 1602 |
| Download times: 654 |
| Abstract: |
| Effects…of…cold…shock…on…8…biochemical…and…6…cellular…immunological…parameters…were…detected…in…
Litopenaeus…vannamei by…biochemical…analysis…and…flow…cytometry…to…investigate…the…cold…tolerance…of…this…species.…The…
results…showed…that， when…water…temperature…decreased…from…30℃…to…16℃…with…a…decreasing…rate…of…4℃/h， serum…glucose…
significantly…decreased， other…biochemical…parameters…in…serum…including…total…protein（TP） ， alanine…aminotransferase
（AST） ， alanine…aminotransferase（ALT） ， cholesterol， triglycerides， lactate…dehydrogenase（LDH）and…creatine…
kinase（CK）activity/level…increased…in…various…degrees.…Total…haemocyte…count（THC） ， reactive…oxygen…species
（ROS）production…and…non-specific…esterase（NSE）activity…significantly…decreased…after…cold…shock， while…apoptotic…
cell…ratio…significantly…increased.…The…above…results…reflected…the…effects…of…cold…shock…on…energy…metabolism， cellular…
membrane…permeability…and…celluar…immunity…of…L. vannamei.…Cold…shock…may…cause…blood…serum…exhausting， enhance…
decomposition…of…protein…and…lipid， increase…cellular…membrane…permeability…and…tissue…injury， metabolizing…enzymes…
leaking…out， and…impaire…celluar…immunity.…It…can…be…concluded…that…L. vannamei…is…very…sensitive…to…cold…shock， which…
may…result…in…physiological…depression…of…the…species. |
|
View Full Text
View/Add Comment Download reader |
