|
| Construction and transformation of GFP fusionexpression vector of rice OsPIN1b gene |
| |
| DOI： |
|
| Hits: 1669 |
| Download times: 714 |
| Abstract: |
| Earlier…research…results…showed…that…rice…auxin…efflux…protein…OsPIN1a…probably…participated…
in…regulating…negative…phototropism…of…rice…roots.…To…explore…the…relationship…between…other…OsPIN…genes…and…
negative…phototropism…of…rice…roots， gene-specific…primers…were…used…to…amplify…OsPIN1b gene…by…RT-PCR…based…
on…the…OsPIN1b sequence…in…GenBank.…Sequencing…result…showed…that…the…full…ORF…was…obtained（1…665…bp）.…
Bioinformation…analysis…showed…that…the…sequence…was…100%…identity…with…the…reported…sequence， the…GC…content…of…
OsPIN1b…ORF…was…64.08%…and…OsPIN1b…encoded…554…amino…acid…residues.…The…OsPIN1b-green fluorescent protein
（gfp）fusion…expression…vector…pCAMBIA-1301-OsPIN1b∷GFP…was…constructed…and…transformed…into…Japonica…
rice…Zhonghua11…by…Agrobacterium tumefaciens.…Molecular…detection…and…GUS…assay…showed…that…the…target…construct…
was…integrated…into…the…genome…of…rice， which…laid…the…foundation…for…further…study…of…the…function…of…OsPIN1b…in…
regulating…negative…phototropism…of…rice…roots. |
|
View Full Text
View/Add Comment Download reader |
