|
| Research on cell disruption and DHA extractionof Aurantiochytrium sp. |
| |
| DOI： |
|
| Hits: 2625 |
| Download times: 1962 |
| Abstract: |
| In…order…to…select…the…best…method…for…cell…disruption…of…Aurantiochytrium…sp.， five…cell…disruption…
methods， including…repeated…freezing…and…melting， ultrasonication， high-speed…homogenization， alkaline…heating…
and…trituration， were…used…to…break…up…the…cell…wall…of…Aurantiochytrium sp.， then…cell…disruption…efficiency…of…
different…methods…and…their…influences…on…oil…extraction…and…oil…composition…were…analyzed…and…compared.…In…order…
to…characterize…the…cell…disruption…efficiency…of…different…methods， cell…number…was…estimated…by…microscope…
observation， and…cell…morphology…was…observed…using…SEM.…In…order…to…explore…the…influences…of…different…cell…
disruption…methods…on…oil…extraction， oil…distribution…was…qualitatively…analyzed…using…Nile…red…staining， then…crude…oil…
was…extracted…by…n-hexane.…In…order…to…explore…the…influences…of…different…cell…disruption…methods…on…oil…composition，
the…specific…composition…of…oils…was…analyzed…using…GC（Gas…Chromatography） .…Results…showed…that： … （1）Alkaline…
heating…had…the…best…effect…on…cell…disruption…for…its…highest…efficiency（95.51%）and…biggest…cell…morphology…
changes.… （2）The…extracted…crude…oil…after…alkaline…heating…was…the…most…and…up…to…61.33%…of…the…dry…biomass…weight.…
（3）Highest…DHA…content…in…oil…was…41.33%…after…alkaline…heating， of…which…DHA…production…was…much…higher…than…
the…other…methods…and…up…to…2.192…g/L.…From…the…perspective…of…high…purity…DHA…production， alkaline…heating…is…the…
most…promising…method…for…cell…disruption…of…Aurantiochytrium sp. |
|
View Full Text
View/Add Comment Download reader |
