Abstrct:Proline plays an important role in plant osmotic regulation, Delta-1-pyrroline-5-carboxylate synthase(P5CS) is a key enzyme in proline of glutamic acid synthesis pathway. In order to study the relationship between P5CS gene families and abiotic stress in maize, the maize seedings were treated with abiotic stress( drought, low temperature and salt stress). The expression levels of ZMPSCS in maize( leaf, root) under different stresses were analyzed by semi-quantitative RT-PCR. The results showed that the excepted ZM02G27230 in the ZMP5CS gene family both up-regulated in the leaf and root by drought stress, but its expression among each member peak appeared in different time. The same treatment of the same gene time point of leaf and root,gene expression was different. Under drought stress,ZM08G14210 expression quantity was the highest. Under salt stress,ZM08G31890 expression quantity was the highest. Under low temperature stress,ZM06G25580 expression quantity was the highest. The results indicated that the ZMP5CS expression was induced by abiotic stress. |