文章摘要
Extraction technology of flavonoids in Kalimeris indica root and its inhibitory Effects on activity of Lipoxygenase of Maojian tea leaves
  
DOI:
Author NameAffiliation
吴桂玲1,罗德尉2 (1. 贵州省黔南民族师范学院化学化工学院贵州 都匀 5580002. 贵州高速黔通建设工程有限公司贵州 都匀 558000) 
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Abstract:
      The optimal extraction conditions of flavonoids in Kalimeris indica root were determined by orthogonal design. The results showed that the optimal conditions were as follows: 70% ( volume fraction) ethanol,extraction time of 2.5 h, 1∶20(g/mL) solid-liquid ratio,the yield of total flavonoids could reach 16.422%. The inhibitory effects of flavonoids from root of K. indica on the activity of lipoxygenase of Maojian tea leaves were also studied,and were compared with the same concentration of BHA, BHT and Vc. The results showed that flavonoids from root of K. indica,BHA, BHT and Vc all had inhibitory effects on the activity of lipoxygenase,IC50 were 34.282 μg/mL, 10.938 mg/mL,9.049 μg/mL and 9.04 μg/mL.
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