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| Detection of gene HDAC1 SNP and its relationship with slaughtering traits in Simmental cattle |
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| Abstract: |
| In this study，we cloned，sequenced and analyzed the regions of Intron 4-Intron 5，Intron 10-Intron 11 and 3'-non of gene Histone deacetylase 1（ HDAC1） on the second chromosomes in 37 Simmental cattles. By the methods of PCR-SSCP and sequencing analysis，we tried to judge and determine their SNP genotypes，and explore the relationship between SNP genotypes and slaughter traits in Simmental cattle. The results indicated that，there was no mutation on the regions of Intron 4-Intron 5 and 3'-non-translation of gene HDAC1 in Simmental cattle，but there was one mutation in the region of Intron 10-Intron 11 of gene HDAC1，and it located on the 110 bp polymorphism site of PCR sequencing result，it was a A/G mutation，the frequency of allele G（ 62%） was more than allele A（38%）. By comparison and analysis，it might be co-related between different allele A/G genotype on region of Intron 10-Intron 11 and backfat thickness（ measured） of slaughtering traits of Simmental cattle. There was significantly lower backfat thickness（ measured） of slaughtering traits in genotype of AA than that in genotype of GA or GG. According to the results，in this 110 bp location of region Intron 10-Intron 11 of gene HDAC1，allele G may perform a positive effect on the backfat thickness（measured） of Slaughtering traits in simmental cattle. |
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