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| Identification and sequence analysis of FPPSgene family in Mangifera indica |
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| DOI：10.16768/j.issn.1004-874X.2016.06.013 |
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| Abstract: |
| Farnesyl pyrophosphate synthase gene（ FPPS，EC∶2.5.1.1） is a critical enzyme of sesquiterpene
synthesis，paly a key role in the process of terpenoid biosynthesis. In this study，focusing on mango FPPS gene，taking
Arabidopsis FPPS gene as reference，homologous peptide sequences of FPPS genes were screened from Carica papaya，
Citrus clementina，C. sinensis，Malus domestica，Musa acuminata，Prunus persica，Pyrus bretschneideri，Vitis vinifera，
Actinidia chinensis，Amborella trichopoda genome data by BLASTP. Total 19 FPPS gene sequences were obtained，
then phylogenetic tree was constructed and bioinformatics analysis was carried out. Phylogenetic tree indicated a farther
relationship between monocots and dicots plants，and dicots fruit trees had a close relationship in evolution. Moreover，
the plants in the same family had higher degree of homology. Bioinformatics analysis showed that，in all proteins above
mentioned，acidic protein was 94.7%，stable protein was 84.2%，signal protein was 5.3%，and leader peptide was
10.5%. Meanwhile，all FPPS proteins were hydrophilic proteins with striking transmembrane domain， all located in
mitochondrion and had active site. |
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