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| Protoplast fusion between nicotine-degrading Bacillusamyloliquefaciens and Pseudomonas marginalis tobreed efficient nornicotine strain |
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| DOI：10.16768/j.issn.1004-874X.2016.06.025 |
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| Abstract: |
| In order to acquire fusant with high nicotine-degrading ability，Bacillus amyloliquefaciens T11 and
Pseudomonas marginalis were used for parent strains. After using lysozyme to break the cell walls of parent strains，
the Bacillus amyloliquefaciens T11 protoplast and heat-inactivated Pseudomonas marginalis protoplast were induced by
40% PEG-6000 for protoplast fusion experiment，then the nicotine-degrading ability of fusant was measured. Through
preliminary screening，176 fusion strains were obtained. After rescreening by nicotine selective culture medium，5
fusion strains with higher nicotine-degrading ability were acquired. Genetic stability analysis of the 5 fusion strains and
comparision with parent strains’ nicotine-degrading rate were further measured. A better fusant A2 with higher nicotinededrading
ability than parent strains was obtained finally，93% nicotine in culture medium was degraded in five days. |
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