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| Polymorphism and association analysis of NKX2-5 genewith shank length in three chickens |
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| DOI：10.16768/j.issn.1004-874X.2016.06.027 |
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| Abstract: |
| Gaojiao chicken，Aijiao chicken and Baiyi black chicken in Guizhou province were used as
experimental subjects in this study. SNPs of NKX2-5 genes were detected in three chicken vareties by PCR-SSCP and
DNA sequencing technologies. The 2 SNPs were detected in the 3 chicken groups，T563C，C675T sites of NKX2-5
gene were missense mutations，The relevance between T563C site of NKX2-5 gene and chicken shank length showed
that，TT genotype was significantly higher than CC genotype among Gaojiao chicken groups（P < 0.05），TT genotype
was significantly higher than CT genetype in Baiyi black chicken groups（P < 0.05），uhile no significant level aruong
3 genotypes in Aijiao chicken. The C675T site of NKX2-5 had only CC and CT genotypes，and there was no significant
level in different genotypes of 3 vareties. |
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