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| Influences of brassinolide synthesis gene DWF1 and DET2 on xylem formation in Populus tomentosa |
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| DOI：10.16768/j.issn.1004-874X.2016.07.009 |
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| Abstract: |
| Brassinolides are important for plants growth and development. Transgenic poplars with brassinolides
biosynthesis genes GhDWF1 and GhDET2 were selected and analyzed about the growth，microstructure of xylem
and cell wall composition. It was found that DWF1 and DET2 had influences on xylem formation and cell wall
composition in Populus tomentosa. Transgenic poplars had higher xylem area，smaller cells and thicker cell walls
than the control，which may affect cell division and lateral expansion. Pits on vesselswere enlarged，which may affect
water transport in poplars. Contents of acidic pectin increased，and contents of cellulose decreased in transgenic
poplars， which may change frame structures of cell wall. Transgenic poplars with GhDET2 had higher lignin content
and high S-lignin ratio， which may cause chemical degradation easily，while transgenic poplars with GhDWF1 had
lower lignin content，higher G/S ratio and smaller S-lignin content . |
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