|
| Cloning and expression of porcine carbonyl reductase1（CBR1） gene |
| |
| DOI：10.16768/j.issn.1004-874X.2016.08.025 |
|
| Hits: 2081 |
| Download times: 277 |
| Abstract: |
| The promoter，exons and introns of porcine carbonyl reductase1 gene（ CBR1） were cloned，and the structure of CBR1 protein was analyzed. The similarity of amino acid was compared with other species and the phylogen of the gene was constructed. The tissues expression of the gene was detected by RT-PCR. The results showed that the promoter of the gene contained 2 875 bp，and typical binding sites of NFκB were predicted. The gene included three exons and two introns，and the formers spanned 289，108 and 437 bp，respectively. The two introns spanned 572 bp and 3 219 bp，respectively. The 5′UTR of 107 bp and 3′UTR of 242 bp were identified.The CDS was encoded by 289 amino acids. The CBR1 protein was hydrophilic，and contained two transmembrane domains but no signal peptide was found. The tertiary structure of the protein was formed by seven α-helixes，seven sheets，and loops. The amino acid sequence of the gene exhibited higher similarity of 84.48% with that of human and sheep. The CBR1 mRNA in the endometrium of Erhualian sows was higher significantly than that of Landrace × large white sows on GD12（ gestation day 12）（ P<0.01）. The gene was expressed with the highest level in kidney tissue and the higher in liver and small bowel tissues of Erhualian on GD12. |
|
View Full Text
View/Add Comment Download reader |
