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| Study on a bacillus biofilm growth defective culture medium |
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| DOI：10.16968/j.issn.1004-894X.2016.09.015 |
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| Abstract: |
| Abstract：To find a culture medium could not support bacillus biofilm formation，the colony and pellicle
morphological differences of Bacillus subtilis stain R31 and 168 in solid and liquid MSgg，NB，LB and BGM medium
were compared to make sure the biofilm formation difference of the two strains. Based on the different growth process
of R31 in liquid MSgg，NB and BGM on shaking cultivation，two culture mediums were designed and named BGM1
and BGDM. The growth curve and process of R31 in BGM1 and BGDM were checked and the colony and pellicle
morphological differences of R31 and 168 developed in BGM1 and BGDM were compared. Finally，the biofilm
morphological differences of B. subtilis strain TR21，B. amyloliquefaciens strain R21g9，Brevibacillus sp. strain L1
and B. mucilaginosus strain L2 in BGM1 and BGDM were studied. Results showed that R31 could develop integrated
pellicle in liquid MSgg，NB，LB and BGM medium，and develop wrinkle colony in solid MSgg，LB and NA medium，but develop smooth colony in solid BGM medium. The biofilm of R31 in these mediums were more robust
than that of 168. The cells of R31 grew freely in MSgg and BGM medium in the early stage (2 h) of cultivation，the
cells attached to each other and linked like a chain in logarithmic phase (4 h)，and the cell morphology looked like a
net in the end of logarithmic phase (8 h)，but R31 cells grew freely in NB culture medium at the whole growth process.
These results showed that nutrition but not cultural condition affected the growth process of R31. So the yeast extract
in BGM was changed as beef extract and the medium named BGM1，and tryptone of BGM1 was changed as peptone
and the medium was named BGDM. R31 and 168 could develop pellicle and colony in BGM，but all couldn’t
develop pellicle and winkle colony in BGDM medium. All the tested bacillus strains could develop integrated pellicle
with different thickness and wrinkle colony in BGM1，but they all could not develop integrated pellicle and wrinkle
colony in BGDM medium. Glycerol or Manganese were added in BGDM could recover the integrated pellicle and
wrinkle spread colony formation of R31. Bacillus strains could not develop normal pellicle and winkle colony in
BGDM medium，and this medium could be used to select the materials. |
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