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| Study on decolorization，deodorization and molecular weight distribution of enzymatic hydrolysis prepared from Paphia undulta |
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| DOI：10.16968/j.issn.1004-894X.2016.09.020 |
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| Abstract: |
| In order to make full use of the clam protein resources，the enzymatic hydrolysate was prepared
by complex enzymes hydrolysis in the paper. Taking the sensory evaluation and protein recovery rate as index，
the powder activated carbon（ Zinc oxide carbon，phosphate carbon），granular activated carbon，diatomite（ 10#，
300#），resin（ LX-32，LSA-821） were selected with single factor test for the decolorization and deodorization
of enzymatic hydrolysate，the optimal adsorption conditions were determined and the best adsorbent was selected
by comprehensive index. With the volume and mass ratio of enzyme solution to resin，flow rate as a single factor to optimize the column chromatography separation condition of the best adsorbent，And the gel chromatography was
used for analyzing molecular weight distribution of enzymatic hydrolysate after decolorization and deodorization.
The results showed that the best dynamic adsorption for decolorization and deodorization was obtained by LX-32
resin. The optimized conditions were as follows: the ratio of enzymatic hydrolysate to resin was 2.5:1，the flow rate
was 5 BV/h，under these conditions，the sensory value was 7.8，the protein recovery rate was 78.70（±0.47）%，
the components of molecular weight ranged from 147 to 4 729 u accounted for 75.21%，which had good quality，and
could be used as the base material of health and nutrition food. |
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