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| Effects of different treatments on efficiency of virus-free seed-stems of sugarcane |
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| DOI：10.16768/j.issn.1004-874X.2016.11.003 |
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| Abstract: |
| In order to find out the most efficient pattern to get virus-free sugarcane，and provide a viable
way to produce healthy sugarcane seedlings. We put seed-stems of sugarcane into different thermostat water baths.
The results revealed that the percentage of germination was higher than 90% when the temperature was lower than
52℃ after 7 days of different hot water baths，and was higher than 60% when the temperature was 52℃ and the
time was 30 minutes，and was lower than 30% in other treatments. When the temperature was above 55℃，58℃
and 61℃，we could get sprouts without ratoon stunting disease，sugarcane mosaic virus and sugarcane yellow leaf
virus respectively，but percentage of germination was lower than 20%. However，if we put the seed-stems in 52℃
thermostat water bath for 30 minutes then cultivated in 38℃ thermostat for one week，we cann’t detect pathogenic
microorganisms in growing points of sugarcane. Then under the best condition，we compared the efficiencies of four
virus-free ways: callus induced from heart leaves，axillary bud cultivated，putting into warm water before planting
and hot water bath combined with meristems of axillary bud cultivated. The first and the forth method were better than others. However，after several times transfers of culture，there were more mutants when the callus induced from heart
leaves. Thus，hot water bath combined with meristems of axillary bud cultivated was the best treatment，which could
cultivate healthy sugarcane seedlings. |
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