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| Differentially expressed gene profile of hot pepper seedling leaves between hybrid and its parental lines |
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| DOI：10.16768/j.issn.1004-874X.2016.11.006 |
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| Abstract: |
| In spite of the great success in utilizing heterosis in hot pepper（ Capsicum annuum L.） breeding and
production，the underlying molecular mechanisms are still not well-understood，which need to be resolved as they
are important subjects in basic biology and agronomy. In this study，mature leaf transcriptomes from the F1 hybrids
（D6×D7 and D7×D6） and their parental lines（ D6，D7） were compared. cDNA libraries were prepared using
the sixth true leaves on plants at seedlings followed by RNA-Seq on a Illumina HiSeq 2000 sequencer. RNA-Seq
generated 0.6 billion raw reads，and 0.44 billion high-quality reads（ 100bp） were obtained after the filtering process.
Statistical analysis of genes with presence/ deletion variations showed that there are 1068（ 6.20%） and 780（ 4.56%）
genes in the“ single parent express consistent type” in the direct（ D6×D7） and reciprocal（ D7×D6） F1 hybrids，
respectively. A large portion of the transcriotomes in the four pepper genotypes belong to this type of genes. Gene
expression profiling analysis indicates that in F1 hybrids（ direct and reciprocal crosses），more genes fit into the nonadditive
expression type compared to the parents. Less than 8% of the genes belong to the the additive expression type.
In the non-additive expression type，a large number of the genes fit into the epistatic dominance expression pattern；
it is 66.08% and 62.96% in direct and reciprocal F1 hybrids，respectively. The expression type of these genes fits into
the partial single parent dominance type. |
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