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| Molecular epidemiological investigation of PEDV and analysis of genetic variation of S gene in South Chinaduring 2011-2015 |
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| DOI：10.16768/j.issn.1004-874X.2016.11.020 |
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| Abstract: |
| The study conducted a large scale molecular epidemiological investigation and five years monitoring
of nine swine herds with the outbreak of diarrhea located in geographically separate regions of south China by
RT-PCR. Porcine intestinal and fecal samples were collected from 18 swine farms in five provinces（ Guangdong
province，Guangxi province，Fujian province，Sichuan province，Jiangsu province） in south China with the
piglets younger than 7 days old that showed watery diarrhea and dehydration. 35 spike（ S） genes were determined
to analyze the genetic diversity and epidemiological features during 2011-2015. The results showed that all PEDV
strains could be divided into two groups，Group1 and Group 2. Group1 had a close relationship with the United
States strains and Taiwan strains，and had four subgroups，G1-1，G1-2，G1-3 and G1-4. Group 2 felled into the
same branch with the previous Chinese isolates from 2004 that had two subgroups，G2-1 and G2-2. The subgroup
G1-2 and G1-3 comprised most of the strains isolated in south China while the subgroup G2-2 comprised the strain isolated in 2004（ JS-2004-2） and eight else isolated in south China in our study. It is remarkably that
clinical morbidity caused by two types of PEDV strains were quite different，strains in G2-2 caused relatively
minor mild clinical manifestations，presenting a smaller mortality and shorter onset period，while strains in
G1-2 and G1-3 is caused an outbreak of acute infectious diseases，showing a larger mortality and longer period
especially PEDV strains in G1-3. |
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