非生物胁迫对水通道蛋白启动子活性的调节作用

宋 顺1; 2; 黄东梅1; 2; 徐碧玉3; 金志强1; 2; 孙 威1; 2; $21; 2; 李敬阳1; 2; 许 奕1; 2; 3

文章摘要

Regulation of abiotic stress on aquaporin promoter activity

DOI：10.16768/j.issn.1004-874X.2016.12.001

Author Name	Affiliation
宋顺1，2，黄东梅1，2，徐碧玉3，金志强1，2，孙威1，2，许桂莺1，2，李敬阳1，2，许奕1，2，3	1. 中国热带农业科学院海口实验站，海南海口 570102； 2. 海南省香蕉遗传改良重点实验室，海南海口 570102； 3. 中国热带农业科学院热带生物技术研究所/ 农业部热带作物生物学与遗传资源利用重点实验室，海南海口 571101

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Abstract:

A 841bp sequence of MaPIP1-2 promoter was isolated from banana（ Musa acuminat L. AAA group cv. Brazilian） through chromosome walking PCR. PLACE analysis indicated that MaPIP1-2 promoter contatined abiotic stress-responsive elements，such as drought-responsive elements and salt-related cis-elements. To investigate the function of the promoter，the plant expression vector with MaPIP1-2 promoter was successfully constructed and transformed into Arabidopsis thaliana. In MaPIP1-2：：GUS-expressing Arabidopsis，the activity of MaPIP1-2 promoter was found to be associated with the drought and salt treatment. The activity of MaPIP1-2 promoter was enhanced with the increasing concentration of salt and mannitol.

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