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| Expression analysis of maize WRKY transcription factor genes under abiotic stress |
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| DOI：10.16768/j.issn.1004-874X.2016.12.003 |
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| Abstract: |
| WRKY proteins are large family of plant-specific transcription factors（ TFs），play essential roles
in regulating plant responses to pathogens and abiotic stress. In this study，we identified three WRKY transcription
factor（ TF） genes（ ZmWRKY14-like，ZmWRKY25-like，ZmWRKY62-like） in maize by using bioinformatics
method. Plant-mPLoc predicted that these three proteins were all localized in nucleus. Real-time qPCR assay
showed that ZmWRKY14-like，ZmWRKY25-like，ZmWRKY62-like expressed in all tested maize tissues，but
had differential expression. The expression levels of ZmWRKY14-like and ZmWRKY62-like in young fruit were
significantly higher than those in other tissues，while the expression level of ZmWRKY25-like gene in leaf，
spike and young fruit was higher than that in other tissues. The expression levels of three genes under different
abiotic stress were also analyzed by real-time qPCR. The expression of ZmWRKY25-like was up-regulated，while
ZmWRKY62-like was down-regulated under salt stress. The expression of ZmWRKY62-like was down-regulated
under drought stress. Under low temperature stress，the expression level of ZmWRKY25-like was up-regulated.
These results indicated that ZmWRKY25-like may be involved into plant response to salt and low temperature
stress，and ZmWRKY62-like may be involved into plant response to drought and salt stress. |
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