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| Study on degrading nicotine in tobacco leaves by nicotine-degrading fusant and its parent strains |
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| DOI：10.16768/j.issn.1004-874X.2017.01.004 |
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| Abstract: |
| In order to reduce the nicotine content in upper leaves of tobacco，two different treatment ways of
foliar spraying and root irrigation were designed with Bacillus amyloliquefaciens T11，Pseudomonas marginalis and
fusant A2. The effects of different bacterial fluid，treatment times and treatment methods on nicotine-degrading
efficiency，strain residence time and the quality of tobacco upper leaves were studied. The results showed that root
irrigation could not achieve the purpose of degrading nicotine in the tobacco upper leaves. And the degradation
of nicotine was better than root irrigation after 3 times of foliar spraying. The degradation rates of nicotine by B.
amyloliquefaciens T11，P. marginalis and fusant A2 were 17.7%，18.5% and 25%，respectively. The effect of
fusant A2 on nicotine degradation was the best. In the GFP tracer experiment，the strains would gradually extinct
after spraying bacteria liquid. After treatment，the quality of tobacco upper leaves was in the appropriate range，
and there was an improved tendency to the quality of tobacco upper leaves. Therefore，under foliar spraying
conditions，the nicotine content in tobacco upper leaves significantly decreased，and the quality of tobacco upper
leaves was improved. |
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