|
| DNA barcodes for identification of closely related species in Camellia |
| |
| DOI：10.16768/j.issn.1004-874X.2017.01.009 |
|
| Hits: 2690 |
| Download times: 1281 |
| Abstract: |
| The Camellia genus containing about 280 species is the only genus with great commercial values
in the family Theaceae. The products of this genus such as seed-oil，ornamental plants and different kinds of tea
are popular in the current market. The classification of species in Camellia genus using molecular methods may
have potential power in finding cryptic species，rearing new variety and controlling the trade involving counterfeit.
DNA barcoding is regarded as a promising tool for species-level identification marked by short，standardized DNA
sequences. In this study，we tested the value of widely used barcoding markers（trnH-psbA and matK）on 64
Camellia species and compared with those of other genera in different family downloaded from NCBI. In all，61 trnHpsbA
sequences and 61 matK sequences were obtained and the pairwise distances，aligned length，variable sites
were calculated. As the consistency of matK sequences was extremely high（98.40%），we mainly focused on further
analysis of trnH-psbA region. In genus level，all 61 species were successfully distinguished from 7 other genera.
When comes to species level within Camellia genus，the discrimination rate was only 13.11%. The results indicate
that trnH-psbA region may have effective ability in genus-level identification，but has weak practicability in specieslevel
discrimination. |
|
View Full Text
View/Add Comment Download reader |
