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| Comparative analysis of oxidative stress between leaf andcallus of Citrus Sinensis L.Osbeck cv. Bingtangduring protoplasts isolation |
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| DOI：10.16768/j.issn.1004-874X.2017.02.008 |
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| Abstract: |
| To identify the mechanisms that citrus protoplasts from different origins hold different regeneration
capacity，O2
- production rate，content of malonaldehyde（ MDA），glutathione（ GSH） ，ascorbic acid（ AsA），
and activity of superoxide dismutase（ SOD），catalase（ CAT） and peroxidase（ POD） were analyzed during the
maceration period. Results showed that both the O2
- production rate and MDA content of recalcitrant leaf mesophyllderived
protoplasts were higher than those of callus protoplast at the same time of protoplast isolation. The change
trend of SOD activity of callus protoplasts was similar to that of O2
- production rate，i.e.，an increasing trend during
the maceration period. However，the SOD activity of leaf protoplasts decreased significantly after 12 h of protoplast
isolation. At the same time after protoplast isolation，the CAT activity of callus protoplasts was significantly higher
than that of leaf protoplasts，and the contents of GSH and AsA of callus protoplasts was also higher than those of leaf
protoplasts. Therefore，citrus protoplasts derived from different tissues had different regeneration capacity，could
be due to the metabolic balance of active oxygen species（ AOS），protective enzyme and antioxidants，i.e.，AOSgeneration
and defense systems in cells. |
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