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| Screening of lignin degradation strain MZ-9 and optimizationof enzyme production conditions in compost |
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| DOI：10.16768/j.issn.1004-874X.2017.02.015 |
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| Abstract: |
| This experiment use the potassium ferricyanide color，guaiacol color and aniline blue（ Azure -
B） fading circle method，and liquid fermentation experiment，measured enzyme activitives of Laccase（ Lac） and
Manganese Peroxidase（ MnP） were as high as 9.53 U/mL，9.53 U/mL，lignin degradation rate was 20.32%，from the
compost. An active strain MZ-9 is screened as a strong lignin degradation strain. Physiological and biochemical tests
and 16S rDNA sequence analysis showed that strain MZ-9 belonged to Bacillus and had 99.2% similar to Bacillus
licheniformis. Enzyme production showed that when the pH value was 8.0，the culture temperature was 37℃，
the inoculation amount was 8% and the culture time was 6 d，the Lac and MnP activities of strain MZ-9 were the
highest，and the degradation rate of lignin was 20.51%. Therefore，strain MZ-9 is an excellent lignin degradation
strain，has a very good development and utilization value in the lignin degradation，and can provide a reliable strain
resource support for the subsequent industrial production. |
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