文章摘要
Isolation,identification and saccharificationlevel of cellulose degrading bacteria
  
DOI:10.16768/j.issn.1004-874X.2017.02.016
Author NameAffiliation
郑 丽1,2,张海鹏2,宋艳培3,刘孟浩3,何时雨1,马旭东1,覃新导4,李伯松1 1. 中国热带农业科学院广州实验站广东 广州 5101402. 华南农业大学农学院广东 广州 510642 3. 华南农业大学材料与能源学院广东 广州 5106424. 中国热带农业科学院海南 海口 571101 
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Abstract:
      In this study,298 bacteria were isolated from cassava microenvironments and 57 strains with cellulase activity were gained by in vitro detection test. Results showed that strains with big hydrolysis circle (the score of 3) accounted for 40.9% and strains with weakly hydrolysis circle(the score of 2 and 1) accounted for 27.9% and 24.6%,respectively. The saccharification level and enzyme activity were tested by DNS method,and we found 10 potential strains with efficient ability of degradation of cellulose,in which strain HWY-3-9 had the best saccharification and enzyme activity, the sugar content was 0.1852 mg/mL and enzyme activity was 0.0012 U/mL, and it was identified as Bacillus amyloliquefaciens.
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