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| Extraction and characteristics of polyphenoloxidase from white shrimp |
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| DOI：10.16768/j.issn.1004-874X.2017.03.020 |
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| Abstract: |
| The optimization of extraction and biological characteristics of polyphenol oxidase（ PPO） of white
shrimp（ Penaeus vannamei） were studied. The experimental factors and levels were determined by single factor
experiments. Response surface methodology was used to explore the effects of solid-liquid ratio，pH and extraction
time on PPO activity. The results showed that the optimal extraction conditions were as follows: the solid-liquid ratio
was 2∶1，pH was 8.0 and the extraction time was 3.0 h. Under the optimal extractions，the PPO activity could reach
94.8 A/min·mL. Regressive model of extracted PPO was significant（ P ＜ 0.0001） and better fitting（ R2=0.9762），
it could be used to predict the PPO extraction. The kinetic equation of enzyme reaction was consistent with Michaelis
Menten equation. The Km was 30.322 mmol/L and Vmax was about 0.281 A/min. Study on properties of PPO could
provide theoretical guidance for white shrimp anti-melanosis technology. |
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