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| Yeast diversity and distribution characteristics ofactivity strains producing extracellular enzymein soil of Lhalu Wetland，Tibet |
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| DOI：10.16768/j.issn.1004-874X.2017.04.015 |
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| Abstract: |
| In this paper，we isolated yeasts from soil of Lhalu Wetland using the dilution method. In addition，
we used 26S rDNA D1/D2 domain sequence analysis and traditional methods to identify isolates and screened
extracelluar enzyme activity strains in petri dish. 83 yeast isolates were identified as belonging to 16 species of
5genera，including Cryptococcus，Candida，Protomyces，Rhodotorula and Cystofilobasidium. R. mucilaginosa
was the most species in soil of the wetland，moreover，the relative abundance of Cryptococcus victoriae and
Cryptococcus aspenensis was also high. Diversity indices showed that yeast isolates in the LhWS6 site were
most abundant，however，that of the LhWS9 site were the lowest. The study showed that human activities
had influences on spatial dynamics of yeast community in Lhalu Wetland. The test also manifested that the
percentage of lipase activity strains was the highest at 25℃，however，the number of strains produced amylase
most at 4℃，up to 49 isolates. Furthermore，the percentage of isolates producing amlyse，pectinase，cellulase
and chitinase was higher in cold condition. Yeasts distributing in soil of Lhalu Wetland were abundant，but insingle site，the kind was few，which indicated that the wetland habitat or human activity could effect population
distribution，and it was important to exploit low temperature-tolerant or exoenzyme activity microorganism in
Qinghai-Tibet plateau. |
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