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| Tissue culture and rapid propagation of Yuetang 03-373，a new sugarcane variety |
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| DOI：10.16768/j.issn.1004-874X.2017.05.003 |
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| Abstract: |
| Experiments were carried out using stem of a new sugarcane variety Yuetang 03-373 as
materials，the best synthetic way of tissure culture was obtained by different exogenous hormones ratio for inducing
dedifferentiation and redifferentiation of callus，inducing germination of shoot-tip and axillary bud，clustered buds
cultivation，multiplication during subculture and rooting. The results showed that for Yuetang 03-373，MS + 2，
4-D mg/L was the optimal medium for callus induction，MS+BA0.5 mg/L + IBA0.01 mg/L was the optimal medium
for buds differentiation from callus induction，MS+ BA1.0 mg/L + IBA 0.04 mg/L was the optimal medium for
germination of shoot-tip and axillary bud，MS + BA1.0 mg/L +IBA0.04 mg/L was the optimal proliferation medium，
and the best medium for rooting was 1/2MS + ABT0.20 g/L + IBA0.05 mg/L + Ac0.5 g/L . Etiolation rates of plants
were in order from low to high among coconut juice，casein hydrolysate，yeast extract and control in the subculture
medium . The survival percentage of transplant was up to 99% with the simpal sand medium，transplanting survival
rate was up to 98% in the soil. |
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