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| Genetic diversity and phylogenetic relationships analysis of Chinese cabbage germplasm resourcesby SRAP and SSR… |
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| DOI：10.16768/j.issn.1004-874X.2017.05.007 |
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| Abstract: |
| SRAP and SSR makers were used to analyze the genetic diversity of Chinese cabbage. 59 pairs
of SRAP and SSR primers were selected from 666 SRAP and 160 SSR primers，which could produce stable and
polymorphic bands. 519 bands were amplified from 41 samples and each primer resulted in 6.2 bands. 171 bands
were polymorphic（ percentage of polymorphic bands was 32.9%）. The value of Nei’s genetic variations indexes of
41 cultivars based on the SRAP and SSR data with the cluster analysis varied from 0.58 to 0.87 and the cultivars could
be divided into two groups. The results showed that Chinese cabbage varieties owned abundant genetic diversities
and most of them were clustered based on the source of cultivars. The investigation showed that SRAP was suitable
for analyzing the genetic distance，while for conserving breeding resources，SSR would be a better choice to ensure
genetic diversity. This study combined with SRAP and SSR markers for the first time to analyze the genetic diversity of
Chinese cabbage，which would play an important role in Chinese cabbage breeding. |
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