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| Isolation and identification of high-efficiency rice straw degrading bacteria |
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| DOI：10.16768/j.issn.1004-874X.2017.05.012 |
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| Abstract: |
| In order to achieve rapid degradation of rice stalk and in situ to field，four kinds of bacteria with
strong ability to degrade rice straw were preliminarily selected from the soil using rice straw powder medium and
Congo red-cellulose medium to observe the colony characteristics and morphology. The variation curve of cellulase
activities of these four bacteria were determined by DNS method. By analyzing the weight loss rate and residue
content of rice straw，two among these bacteria had high ability to degrade. Then using 16SrDNA sequence
analysis，the bacteria was identified. In the end，combining these four bacteria，a complex bacterial strain was
established to degrade rice straw. The results showed that the degradation rates of rice straw treated with XJ1，
XJ2，XJ8 and XJ9 were 78.04%，72.68%，68.48% and 76.58% after 15 days of shaking at 35℃ and 180 r/min.
Strain XJ1 was identified as Bacillus licheniformis and strain XJ9 was identified as megathermal Bacillus subtilis.
Degradation rate of rice straw in the complex bacterial strain reached 43.20% after 3 days，and the rice straw
degradation rate reached 49.76% on the 7th day. |
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