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| Comparison of cell wall degrading enzymes from three different Fusarium oxysporum formae speciales ofSolanaceae |
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| DOI：10.16768/j.issn.1004-874X.2017.05.018 |
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| Abstract: |
| We compared the cell wall degrading enzymes，including polygalacturonase（ PG），pectin methylgalacturonase（
PMG），polygalacturonic acid transeliminase（ PGTE），pectin methyl transeliminase（ PMTE） and
cellulose（ Cx），from three different Fusarium oxysporum formae speciales of Solanaceae as F. oxysporum f. sp.
lycopersici（ FOL），F. oxysproum f. sp. melongenae（ FOM），and F. oxysporum f. sp. capsicum（ FOC）. We found
that the higher to lower enzyme activity order were PG，PMG，PGTE，Cx and PMTE in inoculated eggplant or tomato
in planta，while an order of PG，PGTE，PMG，PMTE and Cx in inoculated capsicum. We also found that PG activity
of FOC was higher than that of FOM or FOL in vitro. There was no significant difference in the activity of PMG，PMTE
and Cx in these three fungal in vitro. Analysis of PG isoenzymes by IEF-PAGE showed that FOL，FOM and FOC could
secrete a total of 13 PGs，with 5，5，and 3 PG isoenzymes，respectively. Each fungus had a specific PG band. These
results will provide useful information for managing Fusarium wilt and insights into pathogenic factors for phytopathology. |
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