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| Feather speed genotyping and early sex identificationon of helmet Guinea Fowl |
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| DOI：10.16768/j.issn.1004-874X.2017.05.020 |
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| Abstract: |
| There is a great gap between Guinea fowl and domestic chickens in the zoological classification
status，Guinea fowl cannot turn anal to identify sex at 1d age，which will directly affect the production and
management process. In order to solve the early sex identification of Guinea fowl，we analyzed the Guinea fowl’s
genetic characteristics of feather speed and the possibility that we established a self-mating feather speed system.
At the same time，we tried to take advantage of CHD-1 gene to identify Guinea fowl early gender. Firstly，
At the age of 1d，we observed Guinea fowl’s feather speed by macroscopic observation，and collected the hair
follicle tissue to analyze the genetic of feather speed differences and program genotype discriminant analysis for
slow feather cock；then，we referenced chicken CHD-1 gene sequence to identify Guinea fowl early gender by
amplificating Guinea fowl follicles DNA. There were no community of slow feather individuals. In addition，sex
determination results were consisted with the positive control of domestic chickens bands，and the consistent
between identification results and the anus’ result in 180 day achieved 100%. There may be differences between
the domestic chicken and Guinea fowl in feather speed’s genetic basis，the group couldn’t establish a Guinea fowl
feather strain through the system selection. Howerve，the results showed that domestic chicken CHD-1 could be
used to identify Guinea fowl’s gender，which indicated that CHD-1 gene between normal chicken and Guinea fowl had the same genetic features，could be used for sex identification of Guinea fowl. We can use this PCR method to
identify Guinea fowls early gender before we establish the rapidly and slowly feathering strains and autosexing of
Guinea fowl. |
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