|
| Study on endopeptidase activity during peanut seeds germination |
| |
| DOI：10.16768/j.issn.1004-874X.2017.06.001 |
|
| Hits: 2385 |
| Download times: 764 |
| Abstract: |
| The activity and isozyme of endopeptidases during peanut seeds germination were detected to
research the activity of endopeptidase affected by different temperatures and inhibitors of metalloprotease EDTA.
In this study，we used the standing boards to germinate peanut seeds，used spectrophotometry to determine the
activity of endopeptidase during the germination of seeds. It was found that the ativity of endopeptidase reached the
maximum at the sixth day. Using SDS-PAGE gel electrophoresis，we found 5 kinds of endopeptidase isoenzymes，
in which there were two kind of metalloprotease of the 6 days of germination，and 7 kinds of endopeptidase
isoenzymes，which there were 4 kind of metalloprotease after 8 days and 10 days of germination. The optimum
temperature of these endopeptidase isozymes was about 40℃. Gelatin electrophoresis results showed that EGTA
had inhibitory effect on two isozymes of metal type at 6 days of germination，but it did not completely inhibit the
activity. |
|
View Full Text
View/Add Comment Download reader |
