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| Micropropagation of Phalaenopsis‘ Liying’ and‘ Hongyun’ |
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| DOI：10.16768/j.issn.1004-874X.2017.08.009 |
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| Abstract: |
| Micropropagation of new cultivars of Phalaenopsis‘ Liying’ and Phalaenopsis‘ Hongyun’ was
investigated by using of peduncle as explant. The results indicated that disinfection time had significant effect on the
induction rate of vegetative shoot，after disinfected with 0.1% HgCl2 for 8 minutes，the induction rate of vegetative
bud was the highest，while exogenous hormones had no significant effect on the induction rate of Phalaenopsis
‘Hongyun’. The concentration of 6-BA，organic additives and the number of bud in an inoculums had significant
influence on bud proliferation. At the concentration of 6-BA being 8 mg/L，the proliferation coefficients of
Phalaenopsis‘ Liying’ and Phalaenopsis‘ Hongyun’ were the highest，accounting for 2.31 and 2.22，respectively.
Supplying coconut milk in proliferation medium was more beneficial for alleviating the browning and enhancing the
proliferation coefficient compared with adding potato or banana. Single bud as an inoculum favored bud proliferation
and enhanced the multiplication coefficient. Exogenous hormone had little effect on the root induction rate，but had
significant effect on the mean number of root and height of plantlet. Seedling height of Phalaenopsis‘ Hongyun’ and
Phalaenopsis‘ Liying’ was the highest and the mean number of root was the most on modified 1/2MS medium with
6-BA 0.1 mg/L + NAA 1 mg/L. By using pine bark as culture substrate，the survival rates of Phalaenopsis‘ Liying’
and Phalaenopsis‘ Hongyun’ were 91.58% and 87.27%，respectively. |
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