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| Identification and bioinformatics analysis of 14-3-3ζ gene from Monochamus alternatus |
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| DOI：10.16768/j.issn.1004-874X.2017.10.012 |
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| Abstract: |
| In order to elucidate the characteristics of Monochamus alternatus，the 14-3-3ζ gene from M.
alternatus called Ma14-3-3ζ was cloned by using cDNA library construction，GenBank（KT970073.1）. The
cloned cDNA of Ma14-3-3ζ is 902 bp in length，which contains a 747 bp open reading frame（ ORF） encoding 248
amino acids with a calculated molecular mass of 28.16 KD and an isoelectric point of 4.73. The results also indicate
that Ma14-3-3ζ show the highest homology with Tenebrio molitor（ 98%），and has 95%-90% amino acid sequence
homology with the 14-3-3ζ from 12 species of insects such as Nilaparvata lugens，M. alternatus and T. molitor on the
same branch in the phylogenetic tree based on insect 14-3-3ζ analysis. |
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