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| Effects of overexpression of H3K9me3 demethylase on the in vitro developmental efficiency of clonedporcine embryos |
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| DOI：10.16768/j.issn.1004-874X.2017.10.016 |
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| Abstract: |
| To explore the effects of overexpression of histone H3K9me3 demethylases KDM4B/KDM4D on the
in vitro developmental efficiency of porcine cloned embryos，KDM4B/KDM4D expression vectors were constructed，
and KDM4B/KDM4D mRNA were obtained by in vitro transcription and were microinjected into cloned pig embryos，
respectively. The results show that injected cloned embryos have a similar H3K9me3 level，cleavage rate and
blastocyst rate with non-injected cloned embryos，but have a significantly higher KDM4B/KDM4D expression level
than non-injected control cloned embryo（ P<0.05），and cloned embryos injected with KDM4B mRNA have a
significantly higher total number of cells per blastocyst than non-injected control cloned embryos（ P<0.05）. These
results suggest that overexpression of histone H3K9me3 demethylases KDM4B can significantly improve the in vitro
developmentral rate of cloned porcine embryos. |
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