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| In vitro propagation of bitter gourd （Momordica charantia Linn.） for field cultivation |
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| DOI：10.16768/j.issn.1004-874X.2017.12.005 |
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| Abstract: |
| The in vitro terminal bud and axillary of bitter gourd（ Momordica charantia Linn.） were used for
tissue culture to establish a high-efficiency regeneration system，and the effects of different hormone combinations
and culture medium on the adventitious bud induction，multiplication and radication for the in vitro of different ages
were studied. The results showed that the in vitro of different ages could be induced well in appropriate induction
media，with longer time needed for the induction of bud for older in vitro. It was found that the most appropriate
induction medium for the adventitious buds induction and multiplication were MS + 6 - BA0.3 mg/L + NAA0.3
mg/L and MS + 6-BA0.1 mg/L；the appropriate radication media was MS + IBA 0.1 mg/L. Under the same culture
condition，the seedlings from tissue culture had smaller plant growth but comparable fruit characteristics，earlier
ripening time and higher uniformity，compared to the seed-grown seedlings，suggesting the importance of improved
cultivation practice with application of tissue culture seedlings in bitter gourd field production. |
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