鹅Caspase-1 基因的克隆与序列分析

刘文俊1; 2; 邱金辉1; 阳佑天3; 黄运茂1; 2; 许丹宁1; 2; 田允波1; 2

文章摘要

Cloning and sequence analysis of Caspase-1 gene in Chinese goose

DOI：10.16768/j.issn.1004-874X.2017.12.022

Author Name	Affiliation
刘文俊1，2，邱金辉1，阳佑天3，黄运茂1，2，许丹宁1，2，田允波1，2	1. 仲恺农业工程学院动物科技学院，广东广州 510225；2. 广东省水禽健康养殖重点实验室，广东广州 510225；3. 佛山科学技术学院医药工程学院，广东佛山 528000

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Abstract:

In order to systematically understand the inflammatory system of goose，the total RNA was extracted from the spleen of Magang goose by Trizol method. The caspase-1 gene of goose was cloned by RT-PCR and the bioinformatics analysis was carried out. Sequencing results showed that the complete ORF region of the go-Caspase-1 gene was 813 bp，encoding 270 amino acids（ GenBank ID NO：MG463109）. Homology analysis showed that the sequence of go-Caspase-1 was homologous to duck and chicken，and its differences with other species were relatively large. Protein functional domain analysis showed that the number and distribution of Caspase-1 protein domains were similar in different species. The three-dimensional structure of protein showed that the structure of go-Caspase-1 was similar to that of chicken and human Caspase-1.

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