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| Diversity analysis of FTO gene and their association with meat quality traits in Huai Pig |
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| DOI：10.16768/j.issn.1004-874X.2018.01.018 |
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| Abstract: |
| PCR-RFLPs with EcoR Ⅰ，Xap Ⅰ and Bsh1236 I restriction enzymes were used to analyze
genetic diversities of three SNPs at the 5′-upstream region（g.-167 T>G），the first intron（c.46-139A>T） and
the third exon（c.594C>G） of the FTO gene，and potential associations between genotypes and three meat quality
traits including intramuscular fat（IMF），backfat thickness（BFT） and loineye area（LEA） were investigated.
The results showed that three SNPs presented certain degrees of diversities with the PIC value being 0.276，0.343
and 0.235，respectively；and the frequencies of observed genotypes conformed to Hardy Weinberg equilibrium
（P>0.05）；for g.-167 T>G SNP，G allele was dominant with the frequency being 0.790，whereas CC genotype was
statistically raised IMF content；for c.46-139A>T SNP，T allele was dominant with the frequency being 0.674，and
TT genotype was statistically decreased BFT comparing toAA genotype；for c.594C>G SNP，G allele was dominant
with the frequency being 0.837，and the SNP were not statistically associated with tested traits. In summary，two SNPs of g.-167 T>G and c.46-139A>T could be potential molecular genetic markers for the meat quality traits in the
population of Huai pigs. |
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