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| Isolation，identification and drug sensitivity of Erysipelothrix rhuriopathiae in a certain region of Guangdong |
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| DOI：10.16768/j.issn.1004-874X.2018.04.021 |
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| Abstract: |
| Erysipelothrix rhuriopathiae from the liver，kidney and spleen tissue of suspected E. rhuriopathiae
swine in a certain region of Guangdong was isolated，then aquired dominant bacteria was identified by 16S rRNA
primer gene sequence，the bacteria of E. rhuriopathiae was tested by toxicity test for SPF mice；ELISA method was
used to test IgM and IgG in the serum of mice，to observe the pathological changes of liver and spleen；drug sensitive
test was used to analyze the drug sensitive effect for five bacteria with nine kinds of antibiotic including penicillin，
ampicillin，streptomycin，cefixime and cefotaxime etc. The results showed that five dominant bacteria were isolated
and identified as E. rhuriopathiae by 16S rRNA and separated culture，the concentrations of IgM and IgG were
obvious higher in the toxicity test group mice serum（ P<0.05），the cells of liver and spleen appeared different degree
degeneration and necrosis in the infected mice. The results of drug sensitivity test showed that five bacteria were
resistance to lincomycin，extrem sensitive to penicillin，ampicillin and cefotaxime，serious sensitive to cefalexin and
medium sensitive to azithromycin and enrofloxacin. |
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