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| Cloning and sequence analysis of Actin gene fragment from Talinum paniculatum Gaertn |
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| DOI：10.16768/j.issn.1004-874X.2018.06.004 |
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| Abstract: |
| In order to provide reference gene for studying the expression pattern of functional genes in Talinum
paniculatum，the cDNA fragment of Actin from Talinum paniculatum；was amplified by RT-PCR，by adopting
degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI
database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was
analyzed by online bioinformatics software. Our result showed that this actin cDNA includes 598 base pairs，encoding
a partial actin with 198 amino acids. Sequence alignments showed that it shares over 85% nucleotide sequence
identity and 86% amino acid sequence identity with other plant actins. This study indicated that a new actin cDNA
fragment was isolated from Talinum paniculatum. This cDNA was named TpActin1 and submitted to GenBank
（accession number: MH33309）. |
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