| In this study, leaves of Chinese dwarf cherry variety Nongda No.5 were collected from greenhouse
cultivated seedlings and tissue cultured seedlings respectively. Bud induction and plant regeneration were studied.
Theoptimum medium for adventitious buds induction of leaves from greenhouse was 1/2MS+IAA 0.1 mg/L+NAA
0.05 mg/L+6-BA 0.8 mg/L, in which all the nutrients were halved.The best medium for adventitious buds induction
of leaves from tissue cultured seedlings was1/2MS+IAA 0.05 mg/L+NAA 0.1 mg/L+6-BA 0.8 mg/L, in which
macro-elements were halved. Moreover, auxiliary factor studies showed that the highest bud induction rate was
achieved with the combination of adding 0.2 mg/L AgNO3 into the medium, full light exposure, turning leaves
upside down and using leaves with petioles. |
