| Using the Rosa chinensis Jacq. in Shihezi area of Xinjiang as the material, the Illumina (MiSeq)
platform was used to carry out 26S rDNA high throughput sequencing analysis and Alpha diversity analysis. The
experiment made use of isolation and purification techniques of culturable yeast (dilution coating method and spore
ejection method), sequencing and identification of 26S rDNA D1/D2 region, carbohydrate fermentation tests, carbon
assimilation tests and related physiological tests. The diversity and phylogenetic analysis of epiphytic yeast (culturable
and non culturable) on R. chinensis Jacq. aimed at laying a foundation for the effective utilization of Xinjiang yeast
resources. It also provided a theoretical basis for exploring the interaction between nectar microorganisms and plants.
The results showed that 41 337 optimized sequences were selected by high throughput sequencing of 26S rDNA and
divided into 3 078 OTU (Operational Taxonomic Unit). The epiphytic yeast community structure was diverse. Then
the epiphytic microorganism community structure was identified as 3 phylum, 10 classes, 13 orders, 22 families, 37
genera and 90 species. The abundance of yeast population were different. Basidiomycota was dominant phylum, which was accounted for 18.9%, among the yeast community. Filobasidium was dominant genera, which was accounted for
12.2%. 4 species culturable yeasts from 3 genera were recognized, including Meyerozyma guilliermondii, Rhodotorula
mucilaginosa, Cryptococcus uzbekistanensis and Cryptococcus albidus. The dominant yeast genera is Cryptococcus. |
