| Mulberry leaf, expecially tender shoot, is rich in γ-aminobutyric acid (GABA). In order to
develop and utilize mulberry leaf γ-aminobutyric acid (GABA) ,mulberry leaf GABA was extracted from mulberry
leaves using pulsed ultrasonic and microwave-assisted extraction method. In this study, the effects of extration
time, material-solvent ratio, extraction temperature, ultrasonic power and microwave power on extraction yield were
investigated through single-factor experiment and box-behnken experiment. The results of single-factor experiment
and Box-Behnken test indicated that the extraction time, material and liquid ratio and extraction temperature had
a greater influence on the extraction of GABA from mulberry leaves. The best parameters of ultrasonic-microwave
collaborative extraction were : material-solvent ratio 1 (g) :34 (mL), microwave irradiation power at 144 W, ultrasonic
power at 50 W, and extraction time at 165 s. With this extraction condition, GABA yield was 5.83 mg/g. This method
has the advantages of high extraction rate and short extraction time. |
