| In this paper, the effect of validamycin A on the free growth and biofilm formation of Bacillus
subtilis R31 was determined, and the possible mechanism of the effect of validamycin A on the free growth of B.
subtilis R31 was preliminarily explored, which provide the basis for improving the effect of prevention and expanding
the objects of prevention and control by mixing of validamycin A and B. subtilis R31. The effect of validamycin A
on the biofilm formation of R31 was measured by the method of crystal violet staining after standard validamycin A
and 60% validamycin A were added into the culture medium of B. subtilis R31 respectively, and also the inhibitory
effect of validamycin A on the free growth of R31 and the counteraction of trehalose on the former were examined by
determination of OD value of fermentation liquid. The rshowed that standard validamycin A and 60% validamycin
A could promote the biofilm formation of R31 at stationary conditions, but inhibit the free growth of R31 at shaking
conditions, and these phenomenon were not related to the production of R31 extracellular polysaccharide; addition of
trehalose can reduce the inhibition. Validamycin A could be mixed with R31 for improving the effect of prevention and
expanding the objects of prevention and control. |
