| The rice spotted leaf mutant Spl34 is a naturally obtained mutant. The mutant Spl34 had been studied in the morphological, physiological and biochemical aspects of the mutant Spl34. The F2 gene localization population was constructed by hybridization of indica rice Francis and Spl34. Genetic analysis showed that the spotted leaf phenotype of Spl34 was regulated by a single dominant gene Spl34(t). The SSR/InDel marker was used to link the leafless leaf recessive plants, and Spl34(t) was finely mapped on chromosome 11 between 23.483 and 23.530 Mb. The length of the localization interval was 46.99 kb, and 8 candidate genes were screened. In addition, high-throughput sequencing technology was used to re-sequence the F2 (Francis/Spl34) spotted individual pool, the spotless individual pool and the parents, and the genomic SNP was used to correlate the spotted and non-spotted pools. Positioned on the 11th chromosome between 23.223-23.791 Mb, the interval size is about 568 kb; further Spl34 mutants were sequenced by single-molecule sequencing technology, and detected by de novo assembly and correlation analysis in two overlapping groups tig00001409 and tig00003011 to the close chain. The length of the new contig generated by Sanger sequencing of the sequences between the two contigs reached 233.95 kb, and the Spl34(t) localization interval was about 40 kb. In this study, the spotted leaf gene Spl34(t) to the 40 kb region of chromosome 11, which laid a good foundation for the further functional study of this gene. |
